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	Provedor de dados BJMBR (2) Autor Fernandes,K.V.S. (2) Oliveira,A.E.A. (2) Silva,L.B. (2) Venâncio,T.M. (2) Xavier-Filho,J. (2) Astolfi Filho,S. (1) Azevedo,C.R. (1) Cavalcante,C.P. (1) Cruz,M.A.L. (1) Machado,O.L.T. (1) Santos,S.S.S. (1) Uchôa,A.F. (1) Mais... Palavra-chave Evolution (2) Insulin (2) Insulin-like (2) Algae (1) Antigens (1) Cowpea (1) Cyanobacteria (1) Developing fruits (1) Fungi (1) Plants (1) Sequence homology (1) Vigna unguiculata (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea) BJMBR Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.. Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004 The leaves of green plants as well as a cyanobacterium, a red alga, and fungi contain insulin-like antigens BJMBR Silva,L.B.; Santos,S.S.S.; Azevedo,C.R.; Cruz,M.A.L.; Venâncio,T.M.; Cavalcante,C.P.; Uchôa,A.F.; Astolfi Filho,S.; Oliveira,A.E.A.; Fernandes,K.V.S.; Xavier-Filho,J.. We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin; Insulin-like; Antigens; Evolution; Plants; Cyanobacteria; Algae; Fungi. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300004 Registros recuperados: 2 Primeira ... 1 ... Última

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